|本期目录/Table of Contents|

[1]吴春燕.复方中药对运动疲劳大鼠海马BDNF及其受体XTrkBmRNA[JP]和蛋白表达的影响[J].山东体育科技,2013,05:0.
 WU Chun-yan.Effects of compound Chinese medicine on the expression of BDNF,TrkB mRNA and protein of rats’hippocampus after exercise-induced fatigue[J].,2013,05:0.
点击复制

复方中药对运动疲劳大鼠海马BDNF及其受体XTrkBmRNA[JP]和蛋白表达的影响

《山东体育科技》[ISSN:1009-9840/CN:37-1011/G8]

期数:
2013年05期
页码:
0
栏目:
出版日期:
2013-10-25

文章信息/Info

Title:
Effects of compound Chinese medicine on the expression of BDNF,TrkB mRNA and protein of rats’hippocampus after exercise-induced fatigue
作者:
吴春燕
山东省体育科学研究中心,山东 济南250101
Author(s):
WU Chun-yan
Shandong Research Center of Sports Scinece,Jinan 250101,China
关键词:
运动疲劳BDNFTrkB复方中药
Keywords:
exercise-induced fatigue BDNFTrkBcompound Chinese medicine
分类号:
G804.7
DOI:
-
文献标识码:
A
摘要:
目的:通过观察复方中药八珍加肉桂补骨脂汤对运动疲劳大鼠海马BDNF及其受体TrkB表达水平的影响,探讨该方药对运动疲劳的神经生物学机制。方法:70只SD大鼠随机分为3组:对照组,模型组,中药组,其中模型组、中药组又各分为力竭后即刻、恢复12 h、恢复24 h三个亚组。模型组和中药组通过7周的递增负荷跑台训练建立运动疲劳模型,用实时荧光定量PCR和免疫组化法检测各组大鼠海马组织力竭后即刻、恢复12 h、恢复24 h三个不同时间点的BDNF、 TrkBmRNA和蛋白表达水平的变化。结果:与对照组相比,模型组大鼠力竭后即刻的BDNF、TrkBmRNA和蛋白表达水平未见明显变化(P>0.05),恢复12 h显著升高,恢复24 h又显著降低(P﹤0.01,P<0.05);中药组大鼠力竭后即刻的BDNF、 TrkBmRNA和蛋白表达水平与模型组无明显差别(P>0.05),恢复12 h显著升高(P<0.01),恢复24 h未见明显下降,且恢复12 h、24 h的mRNA和蛋白表达水平均显著高于模型组(P<0.01)。结论: BDNF及受体TrkB参与了运动疲劳引起的神经系统损伤的修复过程;中药八珍加肉桂补骨脂汤可以通过提高BDNF、TrkB的表达水平,对运动疲劳引起的神经系统损伤起到修复和保护作用。
Abstract:
Objective:To investigate the neurobiological mechanism of compound Chinese medicine on exercise-induced fatigue through observing BDNF and its receptor TrkB expression changes in rats hippocampus after exercise-induced fatigue. Methods:70 healthy rats were randomly divided into control group,model group, and the group of Chinese medicine. The rats in model group and the group of Chinese medicine were also divided into three subgroups: the immediate time point after exhaustion, the time point of 12h recovery and of 24h recovery. The rats of model group and the group of Chinese medicine were established the model of exercise-induced fatigue through seven- week treadmill running of increasing intension. The lever of BDNF,TrkB gene and protein expression at three different time points were measured by Real-time Fluorescent Quantitative PCR and spectrophotometr method.Results:Compared to the control group, the lever of BDNF,TrkB gene and protein expression in model group at immediate time point after exhaustion were not changed obviously, but were increased significantly at the time point of 12h recovery and were decreased significantly at the time point of 24h. Compared to the control group, the lever of BDNF,TrkB gene and protein expression in the group of Chinese medicine at immediate time point after exhaustion were not changed obviously, but were increased significantly at the time point of 12h recovery and 24h recovery. Furthermore the lever of BDNF,TrkB gene and protein expression in the group of Chinese medicine at the time point of 12 h recovery and 24h were significantly higher than in model group. Conclusion:BDNF and its receptor TrkB are involved in the repair process of nervous system damage caused by exercised-induced fatigue; The decoction of cinnamon and scurfpea fruit added to bazhen has a protective effect on nervous system damage caused by exercised-induced fatigue through increasing the BDNF and TrkB expressions.

参考文献/References

[1] Endres M, Fan G, Hirt L,et al.Ischemic brain damage in mice after selectively modifying BDNF or NT4 gene expression[J].[LL] [HJ1.25mm]J Cereb Blood Flow Metab, 2000, 20(1):139-144. [2] Kiprionova I, Sandkuhler J, Schwab S,et al.Brain-derived neurotrophic factor improves long- term potentiation and cognitive functions after transient forebrain ischemia in the rat[J].Exp Neurol,1999, 159(2): 511-519. [3] Castrén E, Rantamaki T. Role of brain-derived neurotrophic factor in the aetiology of depression: implications for pharmacological treatment[J]. CNS Drugs, 2010, 24(1): 1-7. [4]张鹏云,何荣丽,何生等.力竭运动后大鼠海马NF-κB、BDNF的表达变化.神经解剖学杂志,2012,28(1):36~39. [5]吴春燕,吴智春,宋惠霄. 运动疲劳对小鼠海马GDNF及其受体GFR a-1mRNA和蛋白表达的影响[J]. 山东体育学院学报,2010,26(4):42-45. [6]吴春燕,吴智春,宋惠霄. 运动疲劳对小鼠海马GDNF及其受体GFR a-1mRNA表达的影响[J]. 中国运动医学杂志,2011,30(1):53-56. [7]Bedford TG, Tipton CM, Wilson N C,et al.Maximum oxygen consumption of rats and its changes with various experimental procedures[J]. J Appl Physiol, 1979, 47(6): 1278-1283. [8]Antunes PB, Rosa MA, Belmonte-de-Abreu PS,et al.Electrocon-vulsive therapy in major depression: current aspects[J]. Revista Brasileira De Psiquiatria, 2009, 31(Suppl1): S26-S33. [9]Gould E,Tanapat P,Rydel T,Hastings N.Regulation of HIppoeampal Neurogenesis in Adulthood[J].Biol Psyehlatry,2000,48:715-720. [10]Hoshaw BA, Malberg JE, Lucki I. Central administration of IGF-I and BDNF leads to long-lasting antidepressant-like effects[J].Brain Res, 2005, 1037(1-2): 204-208. [11]Ferrer I, Ballabriga J, MartiE,et al.BDNF up-regulates TrkB protein and prevents the death of CAL neurons following transient forebrain ischemia[J].Brain Patho,1998, 8(2): 253-261. [12]Ebendal T.Function and evolution in the family and its receptor[J].JNeurosci Res, 1992, 32: 461-463. [13]Cheng B, Matton M P. NT-3 and BDNF protect CNS neurons against metabolic/excitotoxic insults[J].Brain Res,1994, 640(1-2): 56-67. [14]Kiprianova I, Freiman T M, Desiderato S,et al.Brain-derived neurotrophic factor prevents neuronal death and glial activation after global ischemia in the rat[J].J Neurosci Res,1999,56(1): 21-27. [15]Farmer J,Zhao X,van Praag H,Wodtke K,Gage FH,Christie BR.Effects of voluntary exercise on synaptic plasticity and gene expression in the dentate gylus of adult male Sprague-Dawley rats invo[J].Neuroscience,2004,124 (1):71-79. [16] Hee Han ,D’Costa A, Back S A,et al.BDNF blocks caspase-3 activation in neuratal hypoxia-ischemia[J].Neuro-bid Dis, 2000, 7(1): 38-53. [17] Schabitz W R, Sommer C, Zoder W,et al.Intravenous brain-derived neurotrophic factor reduces infarct size and coun-terregulates Bax and Bcl-2 expression after temporary focal cerebral ischemia[J].Stroke,2000,31(9): 2212-2217.

备注/Memo

备注/Memo:
山东省自然科学基金“GDNF、GFRα-1在运动疲劳大鼠海马中的表达及复方中药的调节作用”(编号:ZR2009CQ032)
更新日期/Last Update: 1900-01-01